DNA Sequencing is now available using an Illumina/Solexa Genome Analyzer, which is capable of generating approximately 50 million raw reads of 36 nt length per full run, of which 50-70% typically pass quality filtering. A full run may be partitioned into 7 channels with individual samples run independently. Due to the short length of reads, this platform is ideal for re-sequencing projects where a reference sequence is available for alignment. If the target sequence is expected to have large insertions/deletions or rearrangements, this technology may not be ideal. The DNA Sequencing and Genotyping laboratory is able to take high-quality dsDNA and perform library preparation, data collection, image analysis, base calling, and alignment to a reference genome using Illumina's eland alignment tool.
For genomic DNA resequencing and other projects that require random shearing of dsDNA prior to library preparation, we require 3 to 5 micrograms of high-quality dsDNA. The best method for quantifying input DNA is a picogreen assay.
For RNA applications, sample preparation can be performed in collaboration with the DNA Microarrays Core Facility.
- Genomic library resequencing
- Amplicon library resequencing
- cDNA library resequencing
- Small RNA discovery and analysis
- Digital Gene Expression
- ChIP-Seq
For additional information about current applications and literature references, visit the Illumina website.
For project consultation, pricing information, or additional information, contact the Director of the DNA Sequencing and Genotyping Lab.